<![CDATA[Scientific Articles - Neurobiology / Neuroscience Feed]]> https://willcowells.com/scientific-articles.html Mon, 06 Apr 2026 17:48:42 +0000 Zend_Feed http://blogs.law.harvard.edu/tech/rss <![CDATA[Live-Cell Quantification of Mitochondrial Functional Parameters]]> https://willcowells.com/scientific-articles/live-cell-quantification-of-mitochondrial-functional-parameters.html Mitochondria are semi-autonomous organelles, which are central to cellular energy production and signal transduction. Given the tight integration between mitochondrial and cellular physiology, experimental strategies are required to study mitochondrial (dys)function in living cells. For this purpose one can use various chemical and protein-based fluorescent reporter molecules (probes), which are introduced into the cell using specific incubation protocols or transfection techniques. These probes include reporters to monitor mitochondrial membrane potential (Δψ), cytosolic and mitochondrial free calcium concentration (Ca2+), reactive oxygen species (ROS), cytosolic and mitochondrial pH, glucose and ATP. However, proper interpretation and quantification of the above readouts is not trivial. Here, we present our protocol for automated temporal analysis of mitochondrial position in living cells and explain how it can be used for computer-assisted quantification of mitochondrial morphology and Δψ. We further discuss how this approach can be applied for simultaneous quantification of multiple mitochondrial and cellular parameters.Images from this Protocol

Source: http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-61779-897-9_6

]]> Sun, 05 Apr 2026 22:00:49 +0000 <![CDATA[Embryo Neuro Stem-Cell]]> https://willcowells.com/scientific-articles/embryo-neuro-stem-cell.html 1 Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, CZ-14220 Prague, Czech Republic.
2 University of Manchester, School of Biological Sciences, Oxford Road, M13 9PT, Manchester, UK
3 Department of Neuroscience, Charles University, Second Medical Faculty, Prague, Czech Republic
4 Institut National de la Santé et de la Recherche Médicale, Unité de recherche U710, Université Montpellier 2,
F-34095 Montpellier cedex 5; and Ecole Pratique des Hautes Etudes, Paris, F-75007, France. Oksana Forostyak,
Department of Molecular signalling, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic,
Prague, Czech Republic.
Nataliya Romanyuk, Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the
Czech Republic, Prague, Czech Republic. kozubenko@biomed.cas.cz
Alexei Verkhratsky, University of Manchester, School of Biological Sciences, Oxford Road, M13 9PT Manchester, UK.
Alexej.Verkhratsky@manchester.ac.uk oxfor@biomed.cas.cz
Eva Sykova, Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech
Republic and Department of Neuroscience, Charles University, Second Medical Faculty, Prague, Czech Republic.
sykova@biomed.cas.cz
Govindan Dayanithi, Department of Molecular Signalling, Institute of Experimental Medicine, Academy of Sciences of
the Czech Republic, Prague, Czech Republic; Institut National de la Santé et de la Recherche Médicale, Unité de
recherche U710, Université Montpellier 2, F-34095Montpellier cedex 5, France; and Ecole Pratique des Hautes Etudes,
Paris, F-75007, France. gdaya@univ-montp2.fr, gdaya@biomed.cas.cz.

Running title: Calcium signalling in human embryonic stem cells.

Key words: stem cells, neuronal population, neuronal markers, molecular signalling, Ca2+ channels, spontaneous Ca2+
oscillations, intracellular Ca2+ stores, ryanodine receptors, ATP, glutamate

Page 1 of 50
Stem Cells and Development Plasticity of Calcium Signalling Cascades in Human Embryonic Stem Cell-Derived Neural
Precursors (doi: 10.1089/scd.2012.0624).

This article has been peer-reviewed and accepted for publication, but has yet to undergo copyediting and proof
correction. The final published version may differ from this proof.

2 Address correspondence to:
Prof. Dr. Govindan Dayanithi, Department of Molecular Signalling, Institute of Experimental Medicine, Academy of
Sciences of the Czech Republic, Videnska 1083, CZ-142 20 Prague 4, Czech Republic.
E-mail :gdaya@univ-montp2.fr ; gdaya@biomed.cas.cz
Phone:+420 241062725, Fax: +420 241062732
web: http://www.iem.cas.cz/research/departments/molecul...




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