<![CDATA[Scientific Articles - Microbiology Feed]]> https://willcowells.com/scientific-articles.html Sat, 11 Apr 2026 15:37:13 +0000 Zend_Feed http://blogs.law.harvard.edu/tech/rss <![CDATA[Quantitative Spatiotemporal Analysis of Phagosome Maturation in Live Cells]]> https://willcowells.com/scientific-articles/quantitative-spatiotemporal-analysis-of-phagosome-maturation-in-live-cells.html Phagocytosis and phagosome maturation are central to the development of the innate and adaptive immune response. Phagosome maturation is a continuous and dynamic process that occurs rapidly. In this chapter, we describe fluorescence-based live cell imaging methods for the quantitative and temporal analysis of phagosome maturation of latex beads and M. tuberculosis as two phagocytic targets. We also describe two simple protocols for monitoring phagosome maturation: the use of the acidotropic probe LysoTracker and analyzing the recruitment of EGFP-tagged host proteins by phagosomes.

Source: http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-4939-6581-6_11

]]> Fri, 10 Apr 2026 22:00:49 +0000 <![CDATA[​Single Cell Analysis of Phagocytosis, Phagosome Maturation, Phagolysosomal Leakage, and Cell Death Following Exposure of Macrophages to Silica Particles]]> https://willcowells.com/scientific-articles/single-cell-analysis-of-phagocytosis-phagosome-maturation-phagolysosomal-leakage-and-cell-death-following-exposure-of-macrophages-to-silica-particles.html Chronic inhalation of silica in various occupational settings results in the development of silicosis, a disease characterized by lung fibrosis. Uptake of silica particles by alveolar macrophages results in cell death and this is one of the contributing factors to the development of silicosis. We have characterized the uncoated or protein-coated (non-opsonized) and Fc receptor-mediated (antibody-opsonized) routes of silica phagocytosis and toxicity. Numerous microscopy techniques and fluorescent probes are outlined in this chapter to carefully measure particle uptake, by macrophages, phagosome maturation, phagosomal reactive oxygen species generation, phagolysosomal leakage, and cell death.

Source: http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-4939-6581-6_5

]]> Tue, 07 Apr 2026 22:05:48 +0000