Application of Atomic Force Microscopy Measurements on Cardiovascular Cells

Sun, 05 Apr 2026 22:00:50 +0000

The atomic force microscope (AFM) is a state-of-the-art tool that can analyze and characterize samples on a scale from angstroms to 100 μm by physical interaction between AFM cantilever tip and sample surface. AFM imaging has been used incrementally over last decade in living cells in cardiovascular research. Beyond its high resolution 3D imaging, AFM allows the quantitative assessments on the structure and function of the underlying cytoskeleton and cell organelles, binding probability, adhesion forces, and micromechanical properties of the cell by "sensing" the cell surface with mechanical sharp cantilever tip. AFM measurements have enhanced our understanding of cell mechanics in normal physiological and pathological states.

Source: http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-61779-523-7_22

Live-Cell Assessment of Mitochondrial Reactive Oxygen Species Using Dihydroethidine

Sun, 05 Apr 2026 22:00:50 +0000

Reactive oxygen species (ROS) play an important role in both physiology and pathology. Mitochondria are an important source of the primary ROS superoxide. However, accurate detection of mitochondrial superoxide especially in living cells remains a difficult task. Here, we describe a method and the pitfalls to detect superoxide in both mitochondria and the entire cell using dihydroethidium (HEt) and live-cell microscopy.

Source: http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-4939-2257-4_15

Scientific Articles - Molecular Medicine Feed

Application of Atomic Force Microscopy Measurements on Cardiovascular Cells

Live-Cell Assessment of Mitochondrial Reactive Oxygen Species Using Dihydroethidine